Material usage types

Detail schemas for material usage rows: one material in one role, as prepared or applied in a procedure, specimen procedure or experimental interface. The role (type) selects the schema.

Devices: Implanted, inserted or attached devices for recording, stimulation, imaging, sensing or fluid delivery, recorded with their sterility and use-specific preparation.
Blood-pressure sensor · Cannula · Catheter · Cranial window · ECoG array · Electrode assembly · Fluid-delivery line · Other device · GRIN lens · Ground electrode · Headpost · Microdialysis probe · Multi-electrode array · Nerve cuff · Optic fiber · Osmotic pump · Patch pipette · Prism · Reference electrode · Respiratory sensor · Silicon probe · Single-wire electrode · Stimulating electrode · Temperature probe or sensor
Components and supporting materials: Hardware and materials that hold, position, protect or fix an implant or specimen, such as cements, fasteners, microdrives, headcaps and slides.
Adhesive, cement, or sealant · Baseplate · Coating · Connector component · Anchor screw, pin, or fastener · Fixed implant carrier or shuttle · Headcap assembly · Manual microdrive · Motorized microdrive · Slide, coverslip, or mounting support · Obturator · Other component · Shielding material
Agents and drugs: Pharmacologically active substances given to a subject or applied to tissue, recorded with the amount of each agent itself rather than of the mixture it was part of.
Active agent · Anesthetic agent · Anticoagulant · Hemostatic agent · Inhaled euthanasia agent · Injected euthanasia agent · Lesion agent · Pharmacological adjunct · Reversal agent · Supportive-care agent
Biological and experimental payloads: Viral vectors, cells, tracers, particles and other components delivered into tissue, recorded with the titer, count, concentration or volume actually delivered.
Cell suspension · Injected component · Neural tracer · Particle suspension · Viral-vector preparation
Fluids and vehicles: Fluids, carrier gases and vehicles that are administered or that carry an active agent, and additives mixed into such fluids.
Administered fluid · Carrier gas · Fluid additive · Irrigation solution · Vehicle
Reagents and labels: Reagents and labels used in histology and specimen processing, from fixation and antigen retrieval through blocking, immunolabeling, staining and clearing.
Blocking reagent · Buffer · Clearing reagent · Dehydration reagent · Detection reagent · Fixative · Hybridization probe · Permeabilization reagent · Primary immunolabeling reagent · Processing reagent · Retrieval reagent · Secondary immunolabeling reagent · Stain or dye · Wash solution
Media and embedding: Media used to process, collect, store, cryoprotect, embed, mount or optically match tissue and specimens.
Cryoprotectant · Embedding medium · Index-matching medium · Processing or collection medium · Mounting medium · Storage medium
Collection and supplies: Containers, additives and disposable supplies used to collect, process or handle a specimen or to support a surgery.
Collection additive · Collection container · Processing supply · Surgical supply
Other: Materials that do not fit any of the specific roles.
Other

Devices

Implanted, inserted or attached devices for recording, stimulation, imaging, sensing or fluid delivery, recorded with their sterility and use-specific preparation.

Blood-pressure sensor

A blood-pressure sensor or telemetry transmitter implanted or attached to measure arterial or venous pressure, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Cannula

A guide or infusion cannula implanted or inserted for drug or fluid delivery, with its sterility and tip preparation.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cut, Beveled, Polished, Other, Unknown.

Catheter

A vascular or other catheter placed for infusion or sampling, with its sterility and preparation of its ends.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`End preparation`	Choice. Final physical preparation of the device end for this use. Options: Factory, Cut, Beveled, Flared, Other, Unknown.

Cranial window

A glass or polymer window placed over a craniotomy for optical access, with its sterility and any surface treatment or coating.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

ECoG array

An ECoG array placed on the cortical surface for electrocorticography, with the number used, sterility, preparation and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Count used`	Integer. Number of arrays used in this record, counted as whole arrays rather than the contacts on them. Range: ≥ 1.
`Preparation`	Text. Use-specific preparation of the array before placement, such as trimming the substrate or soaking.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to the contacts for this use.

Electrode assembly

An assembled set of electrodes, such as a tetrode bundle or multi-wire array, with the number of whole assemblies used, sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Count used`	Integer. Number of assemblies used in this record, counted as whole assemblies rather than the electrodes on them. Range: ≥ 1.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Fluid-delivery line

Tubing or a line connecting a pump or syringe to a cannula or catheter, with its dead, priming and flush volumes, end preparation and sterility.

Field	Description
`Dead volume`	Quantity with unit. Internal volume retained in the configured line. Units: mL, µL, nL.
`Priming volume`	Quantity with unit. Volume used to prime the line before delivery. Units: mL, µL, nL.
`Flush volume`	Quantity with unit. Volume used to flush the line for this use. Units: mL, µL, nL.
`End preparation`	Text. Preparation of the delivery end before use.
`Sterility status`	Choice. Sterility status of the line when used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Other device

A device that does not fit any of the specific device roles, with the number used, sterility, surface treatment and preparation.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

GRIN lens

A gradient-index (GRIN) lens implanted for deep-brain imaging, with its sterility, tip preparation and shape, and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cut, Polished, Other, Unknown.
`Tip shape`	Text. Final shape of the lens tip used.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Ground electrode

An electrode, screw or wire used as the electrical ground for a recording or stimulation, with the number used, sterility, tip preparation and surface treatment.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cut, Stripped, Beveled, Polished, Other, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Headpost

A headpost or head plate fixed to the skull for head restraint, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Microdialysis probe

A microdialysis probe used to sample or deliver substances in extracellular fluid, with its priming volume, membrane preparation, measured relative recovery and sterility.

Field	Description
`Priming volume`	Quantity with unit. Volume used to prime the probe before use. Units: mL, µL, nL.
`Membrane preparation`	Text. Use-specific conditioning or preparation of the dialysis membrane.
`Measured relative recovery`	Number. Measured relative analyte recovery for this probe, expressed as a percentage. Range: ≥ 0 and ≤ 100.
`Sterility status`	Choice. Sterility status of the probe when used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Multi-electrode array

A planar or penetrating multi-electrode array, such as a Utah array or a culture MEA, with its preparation, treatment of the array or contacts, and sterility.

Field	Description
`Preparation`	Text. Use-specific preparation of the array before placement.
`Surface treatment or coating`	Text. Use-specific treatment applied to the array or recording contacts.
`Sterility status`	Choice. Sterility status of the array when used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Nerve cuff

A cuff electrode placed around a peripheral nerve for recording or stimulation, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Optic fiber

An optic fiber implant or patch fiber used for optogenetic stimulation or fiber photometry, with the prepared length, tip preparation and shape, surface treatment and sterility.

Field	Description
`Prepared length`	Quantity with unit. Length of the fiber as prepared for this use, when cut or built in the lab. Units: µm, mm, cm.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cleaved, Polished, Tapered, Other, Unknown.
`Tip shape`	Text. Final shape of the device tip used.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Osmotic pump

An osmotic minipump implanted to deliver a solution continuously over days or weeks.

This type has no type-specific fields. The record’s own fields and its material rows describe it.

Patch pipette

A glass pipette used for patch-clamp or juxtacellular recording, with its tip preparation, such as fire polishing, any coating and sterility.

Field	Description
`Tip preparation`	Choice. Final preparation of the pipette tip for this use. Options: Factory, Pulled, Fire-polished, Coated, Other, Unknown.
`Surface treatment or coating`	Text. Use-specific treatment applied to the pipette surface.
`Sterility status`	Choice. Sterility status of the pipette when used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Prism

A microprism implanted or placed for optical access to a tissue plane, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Reference electrode

An electrode, screw or wire used as the recording reference, with the number used, sterility, tip preparation and surface treatment.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cut, Stripped, Beveled, Polished, Other, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Respiratory sensor

A sensor used to monitor breathing, such as a thermocouple, pressure sensor or chest belt, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Silicon probe

A silicon probe inserted or implanted for extracellular recording, with its sterility and sterilization or disinfection method.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Single-wire electrode

A single-wire electrode used for recording or stimulation, with its tip preparation, surface treatment and sterility.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Tip preparation`	Choice. Final preparation of the device tip for this use. Options: Factory, Cut, Stripped, Beveled, Polished, Other, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Stimulating electrode

An electrode used to deliver electrical stimulation, with its tip preparation, surface treatment and sterility.

Field	Description
`Tip preparation`	Choice. Final preparation of the stimulating surface or tip. Options: Factory, Cut, Stripped, Beveled, Polished, Other, Unknown.
`Surface treatment or coating`	Text. Use-specific treatment applied to the electrode surface.
`Sterility status`	Choice. Sterility status of the electrode when used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Temperature probe or sensor

A thermistor, thermocouple or other probe used to measure body or tissue temperature, with its sterility and any surface treatment.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Components and supporting materials

Hardware and materials that hold, position, protect or fix an implant or specimen, such as cements, fasteners, microdrives, headcaps and slides.

Adhesive, cement, or sealant

A glue, dental or bone cement, or sealant used to secure an implant or seal tissue, with the amount used, mixing ratio, application method and area, and curing method, duration and temperature.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Mixing ratio`	Ratio. Component ratio used to prepare a multi-part material.
`Mixing ratio › numerator`	Required. Number. Range: > 0.
`Mixing ratio › denominator`	Required. Number. Range: > 0.
`Application method`	Text. How this material was applied in this usage.
`Applied area`	Text. Location or surface where this material was applied.
`Curing method`	Choice. Curing, drying, rinsing, or other post-application treatment. Options: Self-cured, Air dried, Heat cured, UV cured, Chemical cure, None, Other.
`Curing duration`	Quantity with unit. Duration allowed for curing, drying, or another post-treatment. Units: d, h, min, s.
`Curing temperature`	Quantity with unit. Temperature used during curing or another post-treatment. Units: °C, K.

Baseplate

A baseplate fixed to the skull or headcap to which a removable device, such as a head-mounted miniature microscope, is attached.

This type has no type-specific fields. The record’s own fields and its material rows describe it.

Coating

A coating applied to a device or component before use, such as a lipophilic dye on a probe shank, with the coated area, application method, concentration and number of coats.

Field	Description
`Application method`	Text. How this material was applied in this usage.
`Coated area`	Text. Part of the device or component that was coated.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Number of coats`	Integer. Number of coating layers applied. Range: ≥ 1.

Connector component

A connector, interface board, adapter or cable used to link an implanted device to the acquisition system, with the number used and its sterility, sterilization or disinfection method.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Anchor screw, pin, or fastener

A bone screw, anchor pin or other fastener used to secure an implant or headcap, with the number and size used, sterility and any preparation.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Size used`	Text. Use-specific size or gauge when it is not already part of the selected catalog or stock identity.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Fixed implant carrier or shuttle

A fixed carrier or shuttle that holds a device at a set position without a movable drive, with how the device is attached, how the carrier is fixed, whether the device can be recovered, and sterility.

Field	Description
`Device can be recovered`	Yes/no. Whether the positioned device can be removed from the carrier for recovery or reuse.
`Device attachment to carrier`	Choice. How the positioned device is attached to the carrier or shuttle. Options: Clamp, Screw, Adhesive, Friction fit, Shuttle or carrier, Socket, Other.
`Carrier fixation method`	Choice. How the carrier or shuttle is secured in its used configuration. Options: Dental cement, Skull screws, Headcap, Bone anchor, Adhesive, Mechanical clamp, Other.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Headcap assembly

A prefabricated or assembled headcap that protects and holds implants on the skull, with its sterility and any surface treatment or coating.

Field	Description
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.

Manual microdrive

A manually adjusted microdrive that advances an implanted device, such as a screw-driven drive for tetrodes or a silicon probe, with drive mechanism, travel range, travel per turn, retractability, recovery, attachment and sterility.

Field	Description
`Drive mechanism`	Text. Manual mechanism used to advance or retract the implanted device.
`Microdrive travel range`	Quantity with unit. Usable range over which the microdrive can move the positioned device. Units: nm, µm, mm.
`Microdrive step size or resolution`	Quantity with unit. Smallest position increment reliably achievable by the microdrive. Units: nm, µm, mm.
`Travel per full turn`	Quantity with unit. Distance the positioned device travels per full turn of the drive screw. Units: nm, µm, mm.
`Device can be retracted`	Yes/no. Whether the microdrive permits the positioned device to be retracted.
`Device can be recovered`	Yes/no. Whether the positioned device can be removed from the microdrive for recovery or reuse.
`Device attachment to microdrive`	Choice. How the positioned device is attached to the microdrive. Options: Clamp, Screw, Adhesive, Friction fit, Shuttle or carrier, Socket, Other.
`Microdrive fixation method`	Choice. How the microdrive is secured in its used configuration. Options: Dental cement, Skull screws, Headcap, Bone anchor, Adhesive, Mechanical clamp, Other.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Motorized microdrive

A motor-driven microdrive that advances an implanted device, with its control mode, travel range, step size, movement rate, retractability, recovery, attachment and sterility.

Field	Description
`Control mode`	Text. How movement of the motorized microdrive is controlled.
`Microdrive travel range`	Quantity with unit. Usable range over which the microdrive can move the positioned device. Units: nm, µm, mm.
`Microdrive step size or resolution`	Quantity with unit. Smallest position increment reliably achievable by the microdrive. Units: nm, µm, mm.
`Microdrive movement rate`	Quantity with unit. Configured movement rate of the motorized microdrive. Units: nm/s, µm/s, mm/s, µm/min, mm/min, µm/h, mm/h.
`Travel per full turn`	Quantity with unit. Distance the positioned device travels per full turn of the drive screw. Units: nm, µm, mm.
`Device can be retracted`	Yes/no. Whether the microdrive permits the positioned device to be retracted.
`Device can be recovered`	Yes/no. Whether the positioned device can be removed from the microdrive for recovery or reuse.
`Device attachment to microdrive`	Choice. How the positioned device is attached to the microdrive. Options: Clamp, Screw, Adhesive, Friction fit, Shuttle or carrier, Socket, Other.
`Microdrive fixation method`	Choice. How the microdrive is secured in its used configuration. Options: Dental cement, Skull screws, Headcap, Bone anchor, Adhesive, Mechanical clamp, Other.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.

Slide, coverslip, or mounting support

A glass slide, coverslip or other support used to mount a tissue section or specimen, with the number used, preparation and application method.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Obturator

A removable insert, such as a dummy cannula or stylet, that keeps a guide cannula or port patent between uses, with the number used, sterility and preparation.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Other component

A supporting component that does not fit any of the specific component roles, with the number used, sterility, surface treatment and preparation.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Surface treatment or coating`	Text. Use-specific surface treatment or coating applied to this device or component.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Shielding material

A material used to shield an implant electrically or optically, such as conductive paint, copper mesh or light-blocking material, with the amount or number of pieces used and how it was applied.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Agents and drugs

Pharmacologically active substances given to a subject or applied to tissue, recorded with the amount of each agent itself rather than of the mixture it was part of.

Active agent

The pharmacologically active substance of a formulation given or applied in a procedure, when no more specific drug role fits, with this agent’s own dose, concentration, dilution, volume or mass.

Field	Description
`Applied dose`	Quantity with unit. This agent’s own dose in this record, normalized to body mass; never the mixture’s combined dose. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. This agent’s own strength in the formulation as prepared, not the formulation’s overall strength. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Ratio by which this agent’s own source material was diluted to prepare it for this use.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume this agent itself contributed here — its own share of any total the parent records. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Absolute amount of this agent contributed here: weighed or fixed, not calculated per body mass. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Prose about preparing this agent here. Row order already records addition order, and a recipe that must be followed belongs in a linked protocol.

Anesthetic agent

An anesthetic drug given for induction or maintenance, with this agent’s own dose, concentration and volume, also when it is one component of a cocktail. A vaporizer setting for an inhaled agent belongs on the event, not here.

Field	Description
`Applied dose`	Quantity with unit. This agent’s own dose in this anesthetic, normalized to body mass; never the cocktail’s total. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. This agent’s own strength in the solution as drawn up; a vaporizer setting belongs on the event. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied volume`	Quantity with unit. Volume this agent itself contributed to the mixture; an inhaled agent has no volume here. Units: L, mL, µL, nL.

Anticoagulant

An anticoagulant such as heparin used to keep blood, catheters or delivery lines from clotting, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Hemostatic agent

A material or agent applied to control bleeding, such as a gelatin sponge, bone wax or topical hemostat, with the amount or number of pieces used and how it was applied.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Inhaled euthanasia agent

A gas or inhalant used for euthanasia, such as CO2 or an overdose of isoflurane, with the applied concentration, flow rate and duration of exposure.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: % v/v, ppm.
`Applied flow rate`	Quantity with unit. Flow rate at which this gas was applied or delivered. Units: L/min, mL/min, µL/min, nL/min.
`Application duration`	Quantity with unit. Duration for which this material was actively applied or delivered. Units: d, h, min, s.

Injected euthanasia agent

An injectable drug used for euthanasia, such as a pentobarbital solution, with the applied dose, concentration, dilution, volume or mass.

Field	Description
`Applied dose`	Quantity with unit. Dose of this material administered or applied in this usage. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Lesion agent

A substance used to produce a chemical or excitotoxic lesion, such as ibotenic acid or 6-OHDA, with the applied dose, concentration, volume and application method.

Field	Description
`Applied dose`	Quantity with unit. Dose of this material administered or applied in this usage. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Pharmacological adjunct

A substance added alongside the main agent to modify its effect or delivery, such as a vasoconstrictor or an enzyme, with its own dose, concentration or activity units per volume and the reason it was added.

Field	Description
`Applied dose`	Quantity with unit. Dose of this substance itself, per unit body mass, not of the mixture it was part of. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration this substance reached in the mixture as applied, after any dilution. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. How far the source material was diluted to prepare what was applied here.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this substance itself that was added, not of the whole mixture. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this substance itself that was added, not of the whole mixture. Units: kg, g, mg, µg, ng.
`Applied activity concentration`	Quantity with unit. Biological activity units of this substance per volume of the mixture as applied, not an infectious titer. Units: IU/mL, U/mL, USP units/mL.
`Purpose`	Choice. Why this substance was added alongside the main agent. Options: Vasoconstriction, pH buffering, Block prolongation, Tissue spread, Irritation reduction, Autonomic counteraction, Sedation deepening, Other.

Reversal agent

A drug given to reverse an anesthetic or sedative, such as atipamezole after medetomidine, with the applied dose, concentration and volume, the dose it was chosen against, and whether reversal was meant to be full or partial.

Field	Description
`Applied dose`	Quantity with unit. Dose of this substance itself, per unit body mass, not of the mixture it was part of. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration this substance reached in the mixture as applied, after any dilution. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied volume`	Quantity with unit. Volume of this substance itself that was administered, not of the whole mixture. Units: L, mL, µL, nL.
`Relative dose basis`	Choice. What this reversal dose was chosen against. Options: Agonist’s dose, Agonist’s volume, Fixed amount per animal, Titrated to effect, Other.
`Purpose`	Choice. Whether reversal was intended to be full or deliberately partial. Options: Full reversal, Partial reversal, Other.

Supportive-care agent

A drug given for supportive or perioperative care, such as an analgesic, anti-inflammatory, antibiotic or ophthalmic ointment, with the applied dose, concentration, dilution, volume or mass.

Field	Description
`Applied dose`	Quantity with unit. Dose of this material administered or applied in this usage. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Biological and experimental payloads

Viral vectors, cells, tracers, particles and other components delivered into tissue, recorded with the titer, count, concentration or volume actually delivered.

Cell suspension

A suspension of cells delivered into tissue, for example for transplantation, with the applied cell count and concentration, viability before application, volume and passage number of cultured cells.

Field	Description
`Applied cell count`	Integer. Total number of cells applied in this usage. Range: ≥ 0.
`Applied cell concentration`	Quantity with unit. Cell concentration in the suspension actually applied. Units: cells/mL, cells/µL.
`Cell viability`	Number. Measured viable-cell percentage immediately before application. Range: ≥ 0 and ≤ 100.
`Applied volume`	Quantity with unit. Volume of cell suspension applied. Units: mL, µL, nL.
`Passage number at use`	Integer. Passage number of cultured cells at the time the suspension was prepared. Range: ≥ 0.
`Preparation`	Text. Use-specific preparation of the cell suspension.

Injected component

One component of an injected or infused formulation when no more specific payload role fits, with its applied dose, concentration, volume, mass or count and its fraction of the final formulation.

Field	Description
`Applied dose`	Quantity with unit. Dose of this material administered or applied in this usage. Units: g/kg, mg/kg, µg/kg, ng/kg.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Fraction of applied formulation`	Number. Fraction of the final applied formulation contributed by this component, entered from 0 to 1 (for example, 0.5 for 50%). Range: ≥ 0 and ≤ 1.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Neural tracer

An anterograde or retrograde tracer delivered into tissue to label neuronal projections, with the applied concentration, dilution, volume or mass and the application method, such as pressure injection or iontophoresis.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of the tracer in the applied formulation. Units: M, mM, µM, nM, mg/mL, µg/mL, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the tracer stock used in the applied formulation.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this tracer preparation applied. Units: mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of tracer applied when that is the reported quantity. Units: mg, µg, ng.
`Preparation`	Text. Use-specific preparation of the tracer.
`Application method`	Text. How the tracer was applied in this usage.

Particle suspension

A suspension of particles, such as fluorescent microspheres or nanoparticles, delivered into tissue, with the applied particle concentration or count, particle size and volume.

Field	Description
`Applied particle concentration`	Quantity with unit. Particle concentration in the suspension actually applied. Units: particles/mL, particles/µL, mg/mL, µg/mL, % w/v, % v/v.
`Applied particle count`	Integer. Total particle count applied when known. Range: ≥ 0.
`Particle size`	Quantity with unit. Nominal or measured particle diameter for this suspension. Units: nm, µm, mm.
`Applied volume`	Quantity with unit. Volume of particle suspension applied. Units: mL, µL, nL.
`Preparation`	Text. Use-specific preparation of the particle suspension.

Viral-vector preparation

A viral vector in the formulation actually injected, with the applied volume, dilution and the effective titer delivered, which can differ from the stock titer.

Field	Description
`Applied volume`	Quantity with unit. Applied volume of this component of the delivered formulation. Units: L, mL, µL, nL.
`Effective delivered titer`	Quantity with unit. Titer of this vector in the formulation actually delivered. Units: vg/mL, gc/mL, TU/mL, IU/mL, pfu/mL, FFU/mL, vp/mL.
`Applied dilution`	Ratio. Dilution used to prepare the delivered formulation.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.

Fluids and vehicles

Fluids, carrier gases and vehicles that are administered or that carry an active agent, and additives mixed into such fluids.

Administered fluid

A fluid given to a subject, such as saline or lactated Ringer’s for fluid support, with the volume of this fluid given.

Field	Description
`Applied volume`	Quantity with unit. How much of this fluid was given, as its own share. It is not the event’s combined total, even when this is the only fluid and the numbers match. Units: L, mL, µL.

Carrier gas

The gas that carries an inhaled anesthetic or is supplied for breathing, such as oxygen or medical air, with the applied flow rate and concentration.

Field	Description
`Applied flow rate`	Quantity with unit. Flow rate at which this gas was applied or delivered. Units: L/min, mL/min, µL/min, nL/min.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: % v/v, ppm.

Fluid additive

A substance added to a fluid, such as drinking water or a perfusion reservoir, with the concentration reached after mixing, the amount added, and whether an equal volume was withdrawn first.

Field	Description
`Applied concentration`	Quantity with unit. Concentration this additive reached in the fluid that was delivered, after mixing. Not the strength of the concentrate or stock it was drawn from. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied volume`	Quantity with unit. Volume of this additive alone that was put into the fluid. Not the volume of the fluid it went into. Units: L, mL, µL.
`Applied mass`	Quantity with unit. Mass of this additive alone that was put into the fluid, when mass rather than volume was the quantity actually measured. Units: kg, g, mg, µg, ng.
`Replacement method`	Choice. Whether an equal volume was withdrawn before this additive went in, which decides whether the stated concentration was actually reached. Options: Equal volume withdrawn first, Added to a full container, Not recorded.

Irrigation solution

A solution used to rinse or keep tissue moist during a procedure, such as sterile saline or aCSF, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Vehicle

The solvent or carrier in which an active agent is dissolved or suspended, such as saline, DMSO or cyclodextrin, with the amount used and its pH, osmolality and sterilization as prepared.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material weighed out for the formulation applied in this record. Units: kg, g, mg, µg, ng.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Ratio by which this material’s own source fluid was diluted to prepare it for this use.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`pH as prepared`	Number. pH of this material as prepared for this use, measured or set by the lab. Range: ≥ 0 and ≤ 14.
`Osmolality as prepared`	Quantity with unit. Osmolality of this material as prepared for this use, read on an osmometer. Units: mOsm/kg.
`Sterilization method as prepared`	Choice. How the lab sterilized this material as prepared for this use. Options: Filter sterilized, Autoclaved, Aseptic handling only, None, Other.
`Preparation`	Text. How this material was made up for this use. Row order already gives the addition order, and a recipe to be followed belongs in a linked protocol.

Reagents and labels

Reagents and labels used in histology and specimen processing, from fixation and antigen retrieval through blocking, immunolabeling, staining and clearing.

Blocking reagent

A reagent used to block nonspecific binding before labeling, such as normal serum or BSA, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Buffer

A buffer used during specimen processing, such as PBS or Tris-buffered saline, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Clearing reagent

A reagent used to render tissue optically transparent, as in CLARITY, iDISCO or CUBIC protocols, with the applied concentration, dilution, volume and application method.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Dehydration reagent

A reagent used to dehydrate tissue, such as a graded ethanol or methanol series, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Detection reagent

A reagent used to detect or amplify a label, such as an avidin-biotin complex, DAB substrate or tyramide amplification reagent, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Fixative

A fixative used to preserve tissue, such as paraformaldehyde for perfusion or immersion fixation, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Hybridization probe

A nucleic acid probe used for in situ hybridization, such as an RNAscope or FISH probe, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Permeabilization reagent

A reagent used to permeabilize tissue or cell membranes for labeling, such as Triton X-100 or saponin, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Primary immunolabeling reagent

A primary antibody or other immunolabeling reagent that binds the target directly, with the working concentration or dilution and volume applied.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Processing reagent

A reagent used in specimen processing when no more specific reagent role fits, with the applied concentration, dilution, volume or mass and application method.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Retrieval reagent

A reagent used for antigen or epitope retrieval, such as citrate buffer for heat-induced retrieval, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Secondary immunolabeling reagent

A secondary antibody or other reagent that binds the primary label, typically conjugated to a fluorophore or enzyme, with the working concentration or dilution and volume applied.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Stain or dye

A histological stain or dye, such as Nissl, DAPI or hematoxylin, with the applied concentration, dilution, volume or mass and application method.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Wash solution

A solution used to rinse tissue between processing steps, such as PBS or PBS with Tween, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Media and embedding

Media used to process, collect, store, cryoprotect, embed, mount or optically match tissue and specimens.

Cryoprotectant

A cryoprotective solution, such as sucrose or glycerol, used to protect tissue before freezing or sectioning, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Embedding medium

A medium in which tissue is embedded for sectioning, such as paraffin, agarose or OCT, with the amount used, preparation and application method.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Index-matching medium

A medium matched to the refractive index of cleared tissue or of the objective for imaging, with the applied concentration, volume, preparation and application method.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Processing or collection medium

A medium used to collect or process tissue or cells when no more specific media role fits, such as culture medium or ice-cold cutting solution, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Mounting medium

A medium used to mount sections under a coverslip, with or without antifade or a nuclear counterstain, with the applied volume, concentration, preparation and application method.

Field	Description
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.
`Application method`	Text. How this material was applied in this usage.

Storage medium

A medium in which tissue or specimens are stored, such as PBS with sodium azide or an antifreeze solution, with the applied concentration, dilution and volume.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Collection and supplies

Containers, additives and disposable supplies used to collect, process or handle a specimen or to support a surgery.

Collection additive

A substance added to a collection tube or medium at collection, such as an anticoagulant or protease inhibitor, with the applied concentration, dilution, volume or mass.

Field	Description
`Applied concentration`	Quantity with unit. Concentration of this material in the formulation or mixture actually applied. Units: M, mM, µM, nM, g/L, mg/mL, µg/mL, ng/mL, mg/L, µg/L, ng/L, % w/v, % v/v.
`Applied dilution`	Ratio. Dilution of the source material used to prepare the applied formulation, expressed as a ratio.
`Applied dilution › numerator`	Required. Number. Range: > 0.
`Applied dilution › denominator`	Required. Number. Range: > 0.
`Applied volume`	Quantity with unit. Volume of this material used in this record. When the parent records a total volume, this is this material’s share of that total. Units: L, mL, µL, nL.
`Applied mass`	Quantity with unit. Mass of this material used in this record. Units: kg, g, mg, µg, ng.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Collection container

A tube, vial, cassette or other container used to collect or hold a specimen, with the number used, sterility and any preparation.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Preparation`	Text. Use-specific preparation of this material, such as mixing, dilution, trimming, or another treatment.

Processing supply

A disposable item used in specimen processing, such as a filter, needle or blade, with the number used, sterility, sterilization or disinfection method and size or gauge.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.
`Sterility status`	Choice. Sterility status of this material when it was used. Options: Sterile, Not sterile, Unknown.
`Sterilization method`	Choice. Method used to sterilize this material. Options: Autoclave, Ethylene oxide, Gamma irradiation, Other, None, Unknown.
`Disinfection method`	Choice. Method used to disinfect this material. Options: Alcohol, Chlorhexidine, Povidone-iodine, Other, None, Unknown.
`Size used`	Text. Use-specific size or gauge when it is not already part of the selected catalog or stock identity.

Surgical supply

A disposable item used during surgery, such as a suture, swab or drape, recorded with the number used.

Field	Description
`Count used`	Integer. Number of items of this material used in this record. Range: ≥ 1.

Other

Materials that do not fit any of the specific roles.