Specimen log types
Entry schemas for specimen logs: measurements followed over time for one specimen set.
Table of contents
- Solution Logs: Logs of bath solutions and culture medium applied to specimens.
Bath solution log · Culture medium change - Environmental Logs: Logs of the conditions around specimens, such as temperature, pH, oxygenation, CO₂ and humidity.
CO₂ log · Humidity log · Oxygenation log · pH log · Temperature log - Health Logs: Logs of specimen health and viability assessments.
Slice health log · Viability assay - Observation Logs: Free-form observations on specimens.
Generic observation
Solution Logs
Logs of bath solutions and culture medium applied to specimens.
Bath solution log
Logs the bath solution applied to a specimen set, such as ACSF, with exchange type, volume, temperature, pH, osmolality, perfusion flow rate, oxygenation gas and the chamber used.
| Field | Description |
|---|---|
Solution name or formulation | Text. Identifies the bath solution, such as ACSF or recovery solution. |
Exchange type | Choice. How the solution was applied or observed. Options: Full exchange, Partial exchange, Continuous perfusion, Measurement only. |
Volume exchanged | Quantity with unit. The volume exchanged or added during this log event. Units: µL, mL, L. |
Solution temperature | Quantity with unit. The solution temperature during the recorded exchange or exposure. Units: °C, K. |
pH | Number. The measured pH of the bath solution. Range: ≥ 0 and ≤ 14. |
Osmolality | Quantity with unit. The measured or declared osmolality of the bath solution. Units: mOsm/kg. |
Perfusion flow rate | Quantity with unit. The perfusion flow rate during this log event. Units: µL/min, mL/min, L/min. |
Oxygenation gas mixture | Text. Gas composition used to oxygenate the solution, for example 95% O₂ / 5% CO₂. |
Device or chamber identifier | Text. The identifier of the device, chamber, or instrument used for the measurement. |
Culture medium change
Logs a change of culture medium, with the replacement medium formulation, volume exchanged and any supplements added.
| Field | Description |
|---|---|
Medium formulation | Text. The formulation or product identity of the replacement culture medium. |
Volume changed | Quantity with unit. The volume exchanged or added during this log event. Units: µL, mL, L. |
Supplements added | Text. Supplements added to the medium during this change, including concentrations when known. |
Environmental Logs
Logs of the conditions around specimens, such as temperature, pH, oxygenation, CO₂ and humidity.
CO₂ log
Logs the CO₂ concentration of the incubator atmosphere holding a culture, with the incubator ID.
| Field | Description |
|---|---|
CO₂ (%) | Number. The carbon-dioxide concentration in the incubator atmosphere. Range: ≥ 0 and ≤ 100. |
Incubator ID | Text. The laboratory identifier of the incubator used for this culture. |
Humidity log
Logs the relative humidity of the chamber atmosphere holding a culture, with the chamber ID.
| Field | Description |
|---|---|
Relative humidity (%) | Number. The relative humidity of the chamber atmosphere as a percentage. Range: ≥ 0 and ≤ 100. |
Chamber ID | Text. The laboratory identifier of the chamber used for this culture. |
Oxygenation log
Logs oxygenation of a bath or chamber, with the applied gas mixture and flow rate, measured dissolved oxygen or partial pressure, and the measurement method, location, device and calibration.
| Field | Description |
|---|---|
Gas composition | Text. Applied gas mixture, for example 95% O₂ / 5% CO₂. |
Gas flow rate | Quantity with unit. The perfusion flow rate during this log event. Units: mL/min, L/min. |
Dissolved oxygen or partial pressure | Quantity with unit. The dissolved-oxygen concentration or oxygen partial pressure at the measurement location. Units: %, mg/L, mmHg. |
Measurement method | Choice. The instrument or method used to obtain this measurement. Options: Applied gas only, Optical sensor, Electrochemical sensor, Blood gas analyser, Other. |
Measurement location | Text. The location within the chamber, bath, or specimen where the value was measured. |
Device identifier | Text. The identifier of the device, chamber, or instrument used for the measurement. |
Calibration reference | Text. Calibration date, certificate, or reference for the oxygen sensor. |
pH log
Logs a pH measurement of a bath, chamber or sample, with measurement temperature, method, location, device and calibration.
| Field | Description |
|---|---|
pH | Number. Measured pH value. Range: ≥ 0 and ≤ 14. |
Measurement temperature | Quantity with unit. The temperature at which the pH measurement was made. Units: °C, K. |
Measurement method | Choice. The instrument or method used to obtain this measurement. Options: pH electrode, Indicator strip, Calculated, Other. |
Sample location | Text. The bath, chamber, or sample location from which the pH value was obtained. |
Device identifier | Text. The identifier of the device, chamber, or instrument used for the measurement. |
Calibration reference | Text. Calibration date, buffers, certificate, or reference for the pH device. |
Temperature log
Logs a temperature measurement in a chamber, bath or specimen, with measurement location, method, device and calibration.
| Field | Description |
|---|---|
Temperature | Quantity with unit. Measured temperature at the stated location and time. Units: °C, K, °F. |
Measurement location | Choice. The location within the chamber, bath, or specimen where the value was measured. Options: Bath, Incubator, Chamber, Specimen surface, Storage, Room, Other. |
Measurement method | Choice. The instrument or method used to obtain this measurement. Options: Temperature probe, Thermocouple, Infrared, Incubator sensor, Other. |
Device identifier | Text. Identifier of the calibrated measurement device when available. |
Calibration reference | Text. Calibration date, certificate, or reference for the measurement device. |
Health Logs
Logs of specimen health and viability assessments.
Slice health log
Logs an assessment of slice health, with overall rating, assessment method and scoring criteria, observed indicators, estimated viability, the context (e.g., before or after recording) and assessor blinding.
| Field | Description |
|---|---|
Overall health rating | Choice. Overall assessment using the stated method and scoring scale. Options: Excellent, Good, Fair, Poor, Non-viable, Not assessed. |
Assessment method | Choice. The observation or assay method used to assess slice health. Options: Visual inspection, Electrophysiology, Imaging, Viability assay, Other. |
Observed indicators | Multi-select. The observed features used to judge slice health. Options: Normal appearance, Torn, Swollen, Opaque, Necrotic, Contaminated, Other. |
Estimated viability (%) | Number. The estimated viable proportion of the assessed cells or tissue as a percentage. Range: ≥ 0 and ≤ 100. |
Scoring scale or criteria | Text. The scoring scale or decision criteria used for the health assessment. |
Assessment context | Text. For example recovery, pre-recording, post-recording, or culture day. |
Assessment blinding | Choice. Whether and how the assessor was blinded during the health assessment. Options: Blinded, Unblinded, Unknown. |
Viability assay
Logs a viability assay on cells or tissue, with the assay type, percent viability and total cell count.
| Field | Description |
|---|---|
Assay type | Text. The assay or measurement method used to estimate viability. |
Viability (%) | Number. The estimated viable proportion of the assessed cells or tissue as a percentage. Range: ≥ 0 and ≤ 100. |
Total cell count | Integer. The total number of cells included in the viability calculation. Range: ≥ 0. |
Notes | Text. A concise qualification of the viability assay result. |
Observation Logs
Free-form observations on specimens.
Generic observation
Logs a free-form observation on a specimen set when no specific log type fits.
This type has no type-specific fields. The record’s own fields and its material rows describe it.
API access
The same types are documented for API users, with property codes, versions and payload examples, on the SpecimenLog schemas API page.