Specimen log types

Entry schemas for specimen logs: measurements followed over time for one specimen set.

Table of contents

Solution Logs

Logs of bath solutions and culture medium applied to specimens.

Bath solution log

Logs the bath solution applied to a specimen set, such as ACSF, with exchange type, volume, temperature, pH, osmolality, perfusion flow rate, oxygenation gas and the chamber used.

FieldDescription
Solution name or formulationText. Identifies the bath solution, such as ACSF or recovery solution.
Exchange typeChoice. How the solution was applied or observed. Options: Full exchange, Partial exchange, Continuous perfusion, Measurement only.
Volume exchangedQuantity with unit. The volume exchanged or added during this log event. Units: µL, mL, L.
Solution temperatureQuantity with unit. The solution temperature during the recorded exchange or exposure. Units: °C, K.
pHNumber. The measured pH of the bath solution. Range: ≥ 0 and ≤ 14.
OsmolalityQuantity with unit. The measured or declared osmolality of the bath solution. Units: mOsm/kg.
Perfusion flow rateQuantity with unit. The perfusion flow rate during this log event. Units: µL/min, mL/min, L/min.
Oxygenation gas mixtureText. Gas composition used to oxygenate the solution, for example 95% O₂ / 5% CO₂.
Device or chamber identifierText. The identifier of the device, chamber, or instrument used for the measurement.

Culture medium change

Logs a change of culture medium, with the replacement medium formulation, volume exchanged and any supplements added.

FieldDescription
Medium formulationText. The formulation or product identity of the replacement culture medium.
Volume changedQuantity with unit. The volume exchanged or added during this log event. Units: µL, mL, L.
Supplements addedText. Supplements added to the medium during this change, including concentrations when known.

Environmental Logs

Logs of the conditions around specimens, such as temperature, pH, oxygenation, CO₂ and humidity.

CO₂ log

Logs the CO₂ concentration of the incubator atmosphere holding a culture, with the incubator ID.

FieldDescription
CO₂ (%)Number. The carbon-dioxide concentration in the incubator atmosphere. Range: ≥ 0 and ≤ 100.
Incubator IDText. The laboratory identifier of the incubator used for this culture.

Humidity log

Logs the relative humidity of the chamber atmosphere holding a culture, with the chamber ID.

FieldDescription
Relative humidity (%)Number. The relative humidity of the chamber atmosphere as a percentage. Range: ≥ 0 and ≤ 100.
Chamber IDText. The laboratory identifier of the chamber used for this culture.

Oxygenation log

Logs oxygenation of a bath or chamber, with the applied gas mixture and flow rate, measured dissolved oxygen or partial pressure, and the measurement method, location, device and calibration.

FieldDescription
Gas compositionText. Applied gas mixture, for example 95% O₂ / 5% CO₂.
Gas flow rateQuantity with unit. The perfusion flow rate during this log event. Units: mL/min, L/min.
Dissolved oxygen or partial pressureQuantity with unit. The dissolved-oxygen concentration or oxygen partial pressure at the measurement location. Units: %, mg/L, mmHg.
Measurement methodChoice. The instrument or method used to obtain this measurement. Options: Applied gas only, Optical sensor, Electrochemical sensor, Blood gas analyser, Other.
Measurement locationText. The location within the chamber, bath, or specimen where the value was measured.
Device identifierText. The identifier of the device, chamber, or instrument used for the measurement.
Calibration referenceText. Calibration date, certificate, or reference for the oxygen sensor.

pH log

Logs a pH measurement of a bath, chamber or sample, with measurement temperature, method, location, device and calibration.

FieldDescription
pHNumber. Measured pH value. Range: ≥ 0 and ≤ 14.
Measurement temperatureQuantity with unit. The temperature at which the pH measurement was made. Units: °C, K.
Measurement methodChoice. The instrument or method used to obtain this measurement. Options: pH electrode, Indicator strip, Calculated, Other.
Sample locationText. The bath, chamber, or sample location from which the pH value was obtained.
Device identifierText. The identifier of the device, chamber, or instrument used for the measurement.
Calibration referenceText. Calibration date, buffers, certificate, or reference for the pH device.

Temperature log

Logs a temperature measurement in a chamber, bath or specimen, with measurement location, method, device and calibration.

FieldDescription
TemperatureQuantity with unit. Measured temperature at the stated location and time. Units: °C, K, °F.
Measurement locationChoice. The location within the chamber, bath, or specimen where the value was measured. Options: Bath, Incubator, Chamber, Specimen surface, Storage, Room, Other.
Measurement methodChoice. The instrument or method used to obtain this measurement. Options: Temperature probe, Thermocouple, Infrared, Incubator sensor, Other.
Device identifierText. Identifier of the calibrated measurement device when available.
Calibration referenceText. Calibration date, certificate, or reference for the measurement device.

Health Logs

Logs of specimen health and viability assessments.

Slice health log

Logs an assessment of slice health, with overall rating, assessment method and scoring criteria, observed indicators, estimated viability, the context (e.g., before or after recording) and assessor blinding.

FieldDescription
Overall health ratingChoice. Overall assessment using the stated method and scoring scale. Options: Excellent, Good, Fair, Poor, Non-viable, Not assessed.
Assessment methodChoice. The observation or assay method used to assess slice health. Options: Visual inspection, Electrophysiology, Imaging, Viability assay, Other.
Observed indicatorsMulti-select. The observed features used to judge slice health. Options: Normal appearance, Torn, Swollen, Opaque, Necrotic, Contaminated, Other.
Estimated viability (%)Number. The estimated viable proportion of the assessed cells or tissue as a percentage. Range: ≥ 0 and ≤ 100.
Scoring scale or criteriaText. The scoring scale or decision criteria used for the health assessment.
Assessment contextText. For example recovery, pre-recording, post-recording, or culture day.
Assessment blindingChoice. Whether and how the assessor was blinded during the health assessment. Options: Blinded, Unblinded, Unknown.

Viability assay

Logs a viability assay on cells or tissue, with the assay type, percent viability and total cell count.

FieldDescription
Assay typeText. The assay or measurement method used to estimate viability.
Viability (%)Number. The estimated viable proportion of the assessed cells or tissue as a percentage. Range: ≥ 0 and ≤ 100.
Total cell countInteger. The total number of cells included in the viability calculation. Range: ≥ 0.
NotesText. A concise qualification of the viability assay result.

Observation Logs

Free-form observations on specimens.

Generic observation

Logs a free-form observation on a specimen set when no specific log type fits.

This type has no type-specific fields. The record’s own fields and its material rows describe it.

API access

The same types are documented for API users, with property codes, versions and payload examples, on the SpecimenLog schemas API page.